Purification and crystallization of glucagon.
نویسندگان
چکیده
Shortly after the discovery of insulin, Kimball and Murlin (1) postulated the existence of a hyperglycemic pancreatic factor, which they named glucagon. The factor was believed to be responsible for the initial transient hyperglycemia following parenteral administration of pancreatic extracts of insulin preparations. Several workers attempted to isolate and purify the active principle. A decade after Kimball and Murlin’s original observations, Biirger and Brandt (2) were able to prepare a partially purified material having glucagon activity. They, however, experienced great difliculties in trying to obtain a substance free from insulin. The investigators hence concluded that insulin and glucagon must be closely related, especially since the chemical properties of the preparations they obtained were very similar to those of insulin itself. In more recent years other workers, Sutherland et al. (3) and de Duve and Vuylsteke (4), obtained small amounts of partially purified preparations having significant glucagon activity. Unfortunately, they were unable to obtain sufficient amounts of material to permit extensive characterization. Our continuing interest in insulin and in its effects on carbohydrate metabolism led us to an interest in this substance, which had an opposite effect to insulin with respect to blood sugar levels. Characterization of the hyperglycemic factor with respect to both physical and biological properties and exploration of possible relationships to insulin necessitated isolation in an essentially pure form. The successful preparation of a crystalline material for which we accepted the name glucagon was announced previously (5) and is reported in detail in this paper.
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 214 2 شماره
صفحات -
تاریخ انتشار 1955